Profile of F2-Isoprostane Level After 5-Day Administration of Robusta Coffee at a Steady State Dose in Subjects Performing Physical Exercise

Authors

  • Rizky Darmawan Master of Sport Health Science, Faculty of Medicine, Universitas Airlangga
  • Izzul Mujahidin Master of Sport Health Science, Faculty of Medicine, Universitas Airlangga
  • Muhammad Fath Alhaqqi Sanis Salamy Master of Sport Health Science, Faculty of Medicine, Universitas Airlangga
  • Ulul Azmy Master of Sport Health Science, Faculty of Medicine, Universitas Airlangga https://orcid.org/0000-0003-2621-9484
  • Gwenny Ichsan Prabowo Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga
  • Prayogi Dwina Angga Elementary School Teacher Study Program, Teacher Training, and Education Faculty, Universitas Mataram, Indonesia
  • Mohd Nahar Azmi Mohamed Department of Sports Medicine, Faculty of Medicine, University Malaya
  • Lilik Herawati Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga

DOI:

https://doi.org/10.47197/retos.v53.102432

Keywords:

coffee, exercise, lipid peroxidation, and F2-isoprostane

Abstract

This study aims to analyze the effect of robusta Dampit coffee on F2-isoprostane levels influenced by ROS due to excessive exercise. This study was an experimental study with a pre- and post-test control group design with coffee administration. The total sample was 18 untrained or sedentary male, divided into two groups: coffee group (COF, n=9) and control/placebo group (PLB, n=9). The COF group was given 2 cups of filtered coffee a day (100 ml/cup) at 12-hour intervals for 5 days. Both groups performed physical exercise with a step test on day 6. Blood samples were collected pre and two hours post exercise. F2-isoprostane concentration was analyzed using the F2-isoprostane biomarker and ELISA to measure the level of lipid peroxidation. Results showed a decrease in F2-isoprostane levels in both COF (pre: 1531.57±278.13 pg/mL; post: 1367.6±230.24 pg/mL; p=0.110) and PLB (pre: 1716.65±501.19 pg/mL; post: 1600.02±500.59 pg/mL; p=0.139) groups with a greater decrease in the COF group. However, this reduction was not significantly different between groups (p=0.734). Although not significantly different, exercising participants tended to have lower F2-isoprostane levels after consuming robusta coffee for five days at a steady state dose. Therefore, further investigation is needed to ascertain the physiological consequences of coffee administration over a longer period of time and its effect on recovery speed.

Keywords: coffee, exercise, lipid peroxidation, and F2-isoprostane

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Published

2024-04-01

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Darmawan, R., Mujahidin, I., Salamy , M. F. A. S., Azmy, U., Prabowo, G. I., Angga, P. D., Mohamed, M. N. A., & Herawati, L. (2024). Profile of F2-Isoprostane Level After 5-Day Administration of Robusta Coffee at a Steady State Dose in Subjects Performing Physical Exercise. Retos, 53, 116–121. https://doi.org/10.47197/retos.v53.102432

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Vol. 53 (2024)

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Original Research Article

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