Association of the MuRF-1/TRIM63 polymorphism with muscle injuries in professional soccer players
DOI:
https://doi.org/10.47197/retos.v57.104261Keywords:
Football, Genetic, Muscle injury, Performance, Single Nucleotide Polymorphism (SNP), Sport genomicAbstract
Muscle injuries are one of the biggest medical problems in professional soccer. Evidence has suggested that genetic polymorphism is a mediating factor in physiological and structural alterations that can lead to muscle injury. The TRIM63 gene polymorphism may affect the MuRF-1 protein, which is vital in the regulation of muscle mass, and is differentially regulated by exercise mode, muscle contraction, and training status. However, central aspects of the relationship between genetic variations, the environment, and muscle injuries still need to be explained. This study aimed to investigate whether MuRF-1/TRIM63 (A/G, rs2275950) was associated with the occurrence of muscle injury in professional soccer players. Forty-six Brazilian soccer players were evaluated. Genomic DNA was extracted using blood samples and semi-structured interviews on muscle injuries were applied after two seasons (2021-2022). Fisher's exact test was used to verify if MuRF-1/TRIM63 was associated with muscle injuries. The MuRF-1/TRIM63 genotypes (c2 = 2.19; p = 0.292), the dominant model (c2 = 1.04; p = 0.299), and the recessive model (c2 = 1.94; p = 0.208) showed no association with muscle injuries in soccer players. Preliminary evidence suggests that this genetic polymorphism may not be a reliable biomarker of muscle injuries in Brazilian professional soccer players.
Keywords: Football; Genetic, Muscle injury, Performance, Single Nucleotide Polymorphism (SNP), Sport genomic.
References
Baehr, L. M., Hughes, D. C., Lynch, S. A., Van Haver, D., Maia, T. M., Marshall, A. G., Radoshevich, L., Impens, F., Waddell, D. S., & Bodine, S. C. (2021). Identification of the MuRF1 skeletal muscle ubiquitylome through quantitative proteomics. Function, 2(4), zqab029. https://doi.org/10.1093/function/zqab029.
Baumert, P., Cocks, M., Strauss, J. A., Shepherd, S. O., Drust, B., Lake, M. J., Stewart, C. E., & Erskine, R. M. (2022). Polygenic mechanisms underpinning the response to exercise‐induced muscle damage in humans: In vivo and in vitro evidence. Journal of Cellular Physiology, 237, 2862-2876. https://doi.org/10.1002/jcp.30723.
Baumert, P., Lake, M. J., Drust, B., Stewart, C. E, & Erskine, R. M. (2017). TRIM63 (MuRF-1) gene polymorphism is associated with biomarkers of exercise-induced muscle damage. Physiological Genomics, 50(3), 142-143. https://doi.org/10.1152/physiolgenomics.00103.2017.
Bodine, S. C., Latres, E., Baumhueter, S., Lai, V. K. M., Nunez, L., Clarke, B. A., Poueymirou, W. T., Panaro, F. J., Na, E., Dharmarajan, K., Pan, Z. Q., Valenzuela, D. M., De Chiara, T. M., Stitt, T. N., Yancopoulos, G. D., & Glass, D. J. (2001). Identification of ubiquitin ligases required for skeletal muscle atrophy. Science, 294(5547), 1704-1708. https://doi.org/10.1126/science.1065874.
Centner, T., Yano, J., Kimura, E., Mcelhinny, A. S., Pelin, K., Witt, C. C., Bang, M. L., Trombitas, K., Granzier, H., Gregorio, C. C., Sorimachi, H., & Labeit, S. (2001). Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. Journal of Molecular Biology, 306(4), 717-726. https://doi.org/10.1006/jmbi.2001.4448.
Chen, S. N., Czernuszewicz, G., Tan, Y., Lombardi, R., Jin, J., Willerson, J. T., & Marian, A. J. (2012). Human molecular genetic and functional studies identify TRIM63, encoding muscle RING finger protein 1, as a novel gene for human hypertrophic cardiomyopathy. Circulation Research, 111(7), 907-919. https://doi.org/10.1161/CIRCRESAHA.112.270207.
Clos, E., Pruna, R., Lundblad, M., Artells, R., & Caussa, J. E. (2019). ACTN3 single nucleotide polymorphism is associated with non-contact musculoskeletal soft-tissue injury incidence in elite professional football players. Knee Surgery Sports Traumatology Arthroscopy, 27(17), 4055–4061. https://doi.org/10.1007/s00167-019-05381-x.
Ekstrand, J., Ueblacker, P., Van Zoest, W., Verheijen, R., Vanhecke, B., Van Wijk, M., & Bengtsson, H. (2023). Risk factors for hamstring muscle injury in male elite football: medical expert experience and conclusions from 15 European Champions League clubs. BMJ Open Sport & Exercise Medicine, 9(1), e001461. https://doi.org/10.1136/bmjsem-2022-001461.
Fagundes, L. H. S., Costa, I. T., Reis, C. P., Pinheiro, G. S., & Costa, V. T. (2021). Monitoring of overtraining and motivation in elite soccer player. Motriz Journal of Physical Education, 27(11), e1021022221. https://doi.org/10.1590/S1980-65742021022221.
Fagundes, L. H. S., Noce, F., Albuquerque, M. R., Andrade, A. G. P., & Costa, V. T. (2019). Can motivation and overtraining predict burnout in professional soccer athletes in different periods of the season? International Journal of Sport and Exercise Psychology, 19(1), 1-16. https://doi.org/10.1080/1612197X.2019.1655778.
Fuller, C. W., Ekstrand, J., Junge, A., Andersen, T. E., Bahr, R., Dvorak, J., Hägglund, M., Mccrory, P., & Meeuwisse, W. H. (2006). Consensus statement on injury definitions and data collection procedures in studies of football (soccer) injuries. Scandinavian Journal of Medicine & Science in Sports, 16(2), 83-92. https://doi.org/10.1111/j.1600-0838.2006.00528.x.
Hägglund, M., Waldén, M., Bahr, R., & Ekstrand, J. (2005). Methods for epidemiological study of injuries to professional football players: developing the UEFA model. British Journal of Sports Medicine, 39(6), 340-346. https://doi.org/10.1136/bjsm.2005.018267.
Harriss, D. J., Jones, C., & MacSween, A. (2022). Ethical standards in sport and exercise science research: 2022 update. International Journal of Sports Medicine, 43(13), 1065-1070. https://doi.org/10.1055/a-1957-2356.
Isık, A., Unlu, G., Gozubuyuk, O. B., Aslanyurek, T., & Bereceli, C. (2018). The relationship between previous lower extremity injury, body weight and bilateral eccentric hamstring strength imbalance in young soccer players. Montenegrin Journal of Sports Science Medicine, 7(2), 23-28. https://doi.org/10.26773/mjssm.180904.
Jackson, A. S., & Pollock, M. L. (1978). Generalized equations for predicting body density of men. British Journal of Nutrition, 40(3), 497-504. https://doi.org/10.1079/bjn19780152.
Larruskain, J., Celorrio, D., Barrio, I., Odriozola, A., Gil, S. M., Fernandez-Lopes, J. R., Nozal, R., Ortuzar, I., Lekue, J. A., & Aznar, J. M. (2018). Genetic variants and hamstring injury in soccer: an association and validation study. Medicine & Science in Sports & Exercise, 50(2), 361-368. https://doi.org/10.1249/MSS.0000000000001434.
Lim, T., Santiago, C., Pareja-Galeano, H., Iturriaga, T., Sosa-Pedreschi, A., Fuku, N., Pérez-Ruiz, M., & Yvert, T. (2021). Genetic variations associated with non-contact muscle injuries in sport: A systematic review. Scandinavian Journal of Medicine & Science in Sports, 31(6), 2014-2032. https://doi.org/10.1111/sms.14020.
López-Valenciano, A., Ruiz-Pérez, I., Garcia-Gómez, A., Vera-Garcia, F. J., Croix, M. D., Myer, G. D., & Ayala, F. 2019. Epidemiology of injuries in professional football: a systematic review and meta- analysis. British Journal of Sports Medicine, 0, 1-9. https://doi.org/10.1136/bjsports-2018-099577.
Maestro, A., Del Coso, J., Aguilar-Navarro, M., Gutiérrez-Hellín, J., Morencos, E., Revuelta, G., Casares, E. R., Perucho, T., & Varillas-Delgado, D. (2022). Genetic profile in genes associated with muscle injuries and injury etiology in professional soccer players. Frontiers in Genetics, 13, 1035899. https://doi.org/10.3389/fgene.2022.1035899.
Mandorino, M., Figueiredo, A. J., Gjaka, M., & Tessitore, A. (2023). Injury incidence and risk factors in youth soccer players: a systematic literature review. Part I: epidemiological analysis. Biology of Sport, 40(1), 3-25. https://doi.org/10.5114/biolsport.2023.109961.
McAuley, A. B. T., Hughes, D. C., Tsaprouni, L. G., Varley, I. Suraci, B., Roos, T. R., Herbert, A. J., Jackson, D. T., & Kelly, A. L. (2022). A systematic review of the genetic predisposition to injury in football. Journal of Science in Sport and Exercise, 5(2), 1-19. https://doi.org/10.1007/s42978-022-00187-9.
Miller, S. A., Dykes, D. D., & Polesky, H. F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16(3), 1215. https://doi.org/10.1093/nar/16.3.1215.
Moya, W. A. (2021). Genética y fútbol: asociación de los polimorfismos genéticos ACTN3 y ACE-I/D en jugadores de fútbol: Revisión literaria. Retos, 39, 929-936. https://doi.org/10.47197/retos.v0i39.79347.
Mueller-Wohlfahrt, H. W., Haensel, L., Mithoefer, K., Ekstrand, J., English, B., Mcnally, S., Orchard, J., Van Dijk, C. N., Kerkhoffs, G. M., Schamasch, P., Blottner, D., Swaerd, L., Goedhart, E., & Ueblacker, P. (2013). Terminology and classification of muscle injuries in sport: The Munich consensus statement. British Journal of Sports Medicine, 47, 342-350. https://doi.org/10.1136/bjsports-2012-091448.
Murtagh, C. F., Hall, E. C., Brownlee, T. E., Drust, B., Williams, A. G., & Erskine, R. M. (2023). The genetic association with athlete status, physical performance and injury risk in soccer. International Journal of Sports Medicine, 44(13), 941-960. https://doi.org/10.1055/a-2103-0165.
Peris-Moreno, D., Taillandier, D., & Polge, C. (2020). MuRF1/TRIM63, Master regulator of muscle mass. International Journal of Molecular Sciences, 21(18), 2-39. https://doi.org/10.3390/ijms21186663.
Pinheiro, G. S., Chiari Quintão, R., Claudino, J. C., Carling, C., Lames, M., & Couto, B. P. (2022). High rate of muscle injury despite no change in physical, physiological and psychophysiological parameters in a professional football team during a long-congested fixture period. Research in Sports Medicine, 31(6), 744-755. https://doi.org/10.1080/15438627.2022.2038159.
Pruna, R., Artells, R., Ribas, J., Montoro, B., Cos, F., Muñoz, C., & Maffulli, N. (2013). Single nucleotide polymorphisms associated with non-contact soft tissue injuries in elite professional soccer players: Influence on degree of injury and recovery time. BMC Musculoskeletal Disorders, 14(1), 1-7. https://doi.org/10.1186/1471-2474-14-221.
Pulici, L., Certa, D., Zago, M., Volpi, P., & Esposito, F. (2022). Injury burden in professional European football (Soccer): Systematic review, meta-analysis, and economic considerations. Clinical Journal of Sport Medicine, 0(0), 1-8. https://doi.org/10.1097/JSM.0000000000001107.
Sinovas, M. C., Hernández, M. L. R., & Cerezal, A. B. (2020). Epidemiology of injuries in young Spanish soccer players according to the playing positions, Retos, 38, 459-464. https://doi.org/10.47197/retos.v38i38.74649.
Stefanetti, R. J., Lamon, S., Wallace, M., Vendelbo, M. H., Russell, A. P., & Vissing, K. (2014). Regulation of ubiquitin proteasome pathway molecular markers in response to endurance and resistance exercise and training. European Journal Physiology, 467(7), 1523-1537. https://doi.org/10.1007/s00424-014-1587-y.
Uchamocha, F. A. P., Cetina, N. F. G., Suescún, C., Ojeda, S. P. C., & Puerto, C. A. C. (2024). Relación entre lesiones musculares com los valores de creatina quinasa y la salud oral en um equipo de fútbol de primera división em Colombia. Retos, 54, 499-505. https://doi.org/10.47197/retos.v54.101008.
Waldén, M., Mountjoy, M., Mccall, A., Serner, A., Massey, A., Tol, J. L., Bahr, R., D’Hooghe, M., Bittencourt, N. F. N., Della Vila, F., Dohi, M., Dupont, G., Fulcher, M., Van Rensburg, D. C. J., Lu, D., & Andersen, T. E. (2023). Football-specific extension of the IOC consensus statement: methods for recording and reporting of epidemiological data on injury and illness in sport 2020. British Journal of Sports Medicine, 0, 1-10. https://doi.org/10.1136/bjsports-2022-106405.
Yang, M-G., Zhang, Q., Wang, H., Ma, X., Ji, S., Li, Y., Xu, L., Bi, Z., & Bu, B. (2022). The accumulation of muscle RING finger-1 in regenerating myofibers: Implications for muscle repair in immune-mediated necrotizing myopathy. Frontiers in Neurology, 24(13). https://doi.org/10.3389/fneur.2022.1032738.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Retos
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and ensure the magazine the right to be the first publication of the work as licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of authorship of the work and the initial publication in this magazine.
- Authors can establish separate additional agreements for non-exclusive distribution of the version of the work published in the journal (eg, to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Is allowed and authors are encouraged to disseminate their work electronically (eg, in institutional repositories or on their own website) prior to and during the submission process, as it can lead to productive exchanges, as well as to a subpoena more Early and more of published work (See The Effect of Open Access) (in English).
This journal provides immediate open access to its content (BOAI, http://legacy.earlham.edu/~peters/fos/boaifaq.htm#openaccess) on the principle that making research freely available to the public supports a greater global exchange of knowledge. The authors may download the papers from the journal website, or will be provided with the PDF version of the article via e-mail.