Increasing running volume elicits hematological changes in trained endurance runners: a case study (El aumento del volumen de entrenamiento de carrera induce alteraciones hematológicas en corredores entrenados: un estudio de caso)


  • José Augusto Rodrigues dos Santos Universidade do Porto


Palabras clave:

endurance, training, running, hemogram, leucogram (resistencia aerobia, entrenamiento, carrera, hemograma, leucograma)


Abstract. Background: Endurance running training induces several hematological changes that increase the capture, transport and delivery of the oxygen to the exercising muscles. Objective: This study aimed to verify how a dramatic increase in running volume induced new alterations in several hematological indicators in previously trained endurance runners. Methods: Three subjects (PL: 26 years, 169,5 cm; HP, 27 years, 167,9 cm; MC, 27 years, 180,7 cm) running 10-12 km/day, increased their running volume to prepare the participation in a 100-km ultra-marathon. New training program included 10-12 training sessions per week, totalizing 200-260 km. Average daily running volume was 35.8±6.2 km. The parameters analyzed were: hemoglobin, erythrocytes, hematocrit, mean cell volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), leukocytes, neutrophils, eosinophils, lymphocytes, and monocytes.  Results: Erythrocyte count, hemoglobin, and hematocrit decreased 6.5%, 5.1% and 6.7%, respectively for the average of the three runners. Leukocytes, neutrophils, eosinophils, lymphocytes, and monocytes showed different alterations among participants with all the values remaining within normal reference values. Conclusion: Well-trained runners show further hematological alterations when training volume is dramatically increased, which can be seen as the specific adaptation to the new training level. It seems that hemogram is more sensitive than leucogram to the increase in running volume.

Resumen. Antecedentes: El entrenamiento de resistencia aeróbica induce varios cambios hematológicos que aumentan la captura, el transporte y la entrega del oxígeno a los músculos en ejercicio. Objetivo: El objetivo de este estudio fue verificar cómo un aumento dramático en el volumen de corrida indujo nuevas alteraciones en varios indicadores hematológicos en corredores previamente entrenados. Métodos: Tres sujetos (PL: 26 años, 169,5 cm, HP, 27 años, 167,9 cm, MC, 27 años, 180,7 cm) corriendo 10-12 km / día, aumentaron su volumen de carrera para preparar la participación en un ultra maratón de 100 km. El nuevo programa de entrenamiento incluyó 10-12 sesiones de entrenamiento por semana, totalizando 200-260 km. El promedio diario de volumen de carrera fue de 35,8 ± 6,2 km. Los parámetros analizados fueron hemoglobina, eritrocitos, hematocrito, volumen celular medio (MCV), hemoglobina corpuscular media (MCH), concentración media de hemoglobina corpuscular (MCHC), leucocitos, neutrófilos, eosinofilos, linfocitos y monocitos. Resultados: El recuento de eritrocitos, la hemoglobina y el hematocrito disminuyeron 6,5%, 5,1% y 6,7%, respectivamente, para el promedio de los tres corredores. Los leucocitos, neutrófilos, eosinofilos, linfocitos y monocitos mostraron diferentes alteraciones entre los participantes con todos los valores restando dentro de los valores normales de referencia. Conclusión: Corredores bien entrenados muestran alteraciones hematológicas adicionales cuando el volumen de entrenamiento aumenta drásticamente, lo que puede ser visto como la adaptación específica al nuevo nivel de entrenamiento. Parece que el hemograma es más sensible que el leucograma al aumento en el volumen de entrenamiento de carrera.

Biografía del autor/a

José Augusto Rodrigues dos Santos, Universidade do Porto

Professor Associado com Agregação

Doutorado em Biologia do Desporto

Departamento de Atletismo 

Especialista em treino desportivo, nutrição e bioquímica do exercício


Banfi G, Lundby C, Robach P, Lippi G (2011). Seasonal variations of haematological parameters in athletes. Eur J Appl Physiol 111: 9-16.

Banfi G, Roi GS, Dolci A, Susta D (2004). Behaviour of hematological parameters in athletes performing marathons and ultramarathons in altitude (“skyrunners”). Clin Lab Haematol 26: 373-7.

Berger NJ, Campbell IT, Wilkerson DP, Jones AM (2006). Influence pf acute plasma volume expansion on VO2 kinetics, VO2peak, and performance during high-intensity cycle exercise. J Appl Physiol 101: 707-14.

Broadbent S (2011). Seasonal changes in haematology, lymphocyte transferring receptors and intracellular iron in Ironman triathletes and untrained men. Eur J Appl Physiol 111: 93 -100.

Brun JF, Varlet-Marie E, Connes P, Aloulou I (2010). Hemorheological alterations related to training and overtraining. Biorheology 47: 95-115.

Burgomaster KA, Howarth KR, Phillips SM, Rakobowchuk M, MacDonald MJ, McGee SL, Gibala MJ (2008). Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. J Physiol 586:151-60.

Cheuvront SN, Haymes EM (2001) Thermoregulation and marathon running: biological and environmental influences. Sports Med, 31(10):743-762

El-Sayed MS, Ali N, Al-Bayatti M (2009). Aerobic power and the main determinants of blood rheology: is there a relationship? Blood Coagul Fibrinolysis 20: 679-85.

El-Sayed MS, Ali N, El-Sayed Ali Z (2005). Haemorheology in exercise and training. Sports Med 35: 649-70.

Fallon KE, Sivyer G, Sivyer K, Dare A (1999). Changes in haematological parameters and iron metabolism associated with a 1600 kilometre ultramarathon. Br J Sports Med 33: 27 -31.

Gjovaag TF, Dahl HA (2008). Effects of training with different intensities and volumes on muscle fibre enzyme activity and cross sectional area in the m. triceps brachhi. Eur J Appl Physiol 103: 399-409.

Gleeson M (1991). Immune function in sport and exercise. J Appl Physiol 103: 693-9.
Helenius I, Lumme A, Haahtela T (2005). Asthma, airway inflammation and treatment in elite athletes. Sports Med 35: 565-74.

Gleeson M, Walsh NP (2012) The BASES expert statement on exercise, immunity, and infection. J Sports Sci, 30(3):321-324

Green RL, Kaplan SS, Rabin BS, Stanitski CL, Zdziarski U (1981) Immune function in marathon runners. Ann Allergy, 47(2):73-75

Hu M, Finni T, Sedliak M, Zhou W, Alen M, Cheng S (2008). Seasonal variation of red blood cell variables in physically inactive men: effects of strength training. Int J Sports Med 29: 564-8.

Kanda K, Sugama K, Hayashida H, Sakuma J, Kawakami Y, Miura S, Yoshioka H, Mori Y, Suzuki K (2013) Eccentric exercise-induced delayed-onset muscle soreness and changes in markers of muscle damage and inflammation. Exerc Immunol Rev, 19:72-85

Kehat I, Shupak A, Goldenberg I, Shoshani O (2003). Long-term hematological effects in Special Forces trainees. Mil Med 168: 116-9.

Knechtle B, Duff B, Schulze I, Hohler G (2008) A multi-stage ultra-endurance run over 1,200 km leads to a continuous accumulation of total body water. J Sports Sci Med, 7(3):357-364

Knechtle B, Knechtle P, Rosemann T, Lepers R (2010). Predictor variables for a 100-km race time in male ultra-marathoners. Percept Mot Skills 111: 681-93.

Kratz A, Lewandrowski KB, Siegel AJ, Chun KY, Flood JG, Van Cott EM, Lee-Lewandrowski E (2002). Effect of marathon running on hematologic and biochemical laboratory parameters, including cardiac markers. Am J Clin Pathol 118: 856-63.

Kratz A, Wood MJ, Siegel AJ, Hiers JR, Van Cott EM (2006). Effects of marathon running on platelet activation markers: direct evidence for in vivo platelet activation. Am J Clin Pathol 125: 296-300.

Lamprecht M, Frauwallner A (2012) Exercise, intestinal barrier dysfunction and probiotic supplementation. Med Sport Sci, 59:47-56

Lehmann M, Wieland H, Gastmann U (1997). Influence of an unaccustomed increase in training volume vs intensity on performance, haematological and blood-chemical parameters in distance runners. J Sports Med Phys Fitness 37: 110-6.

Lombardi G, Ricci C, Banfi G (2011) Effect of Winter swimming on hematological parameters. Biochem Med (Zagreb), 21(1):71-78

Malcovati L, Pascutto C, Cazzola M (2003) Hematologic passport for athletes competing in endurance sports: a feasibility study. Haematologica, 88(5):570-581

Mastaloudis A, Traber MG, Carstensen K, Widrick JJ (2006) Antioxidants did not prevent muscle damage in response to an ultramarathon run. Med Sci Sports Exerc, 38(1):72-80

Morgado JM, Rama L, Silva I, de Jesus Inácio M, Henriques A, Laranjeira P, Pedreiro SA, Rosado F, Alves F, Gleeson M, Pais ML, Paiva A, Teixeira AM (2012) Cytokine production by monocytes, neutrophils, and dendritic cells is hampered by long-term intensive training in elite swimmers. Eur J Appl Physiol, 112(2):471-482

Mujika I, Padilla S, Geyssant A, Chatard JC (1998). Hematological responses to training and taper in competitive swimmers: relationship with performance. Arch Physiol Bioch 105: 379 -85.
Mujika I, Goya A, Padilla S, Grijalba A, Gorostiaga E, Ibañez J (2000) Physiological responses to a 6-d taper in middle-distance runners: influence of training intensity and volume. Med Sci Sports Exerc, 32(2):511-517

Nieman DC, Bucley KS, Henson DA, Warrwn BJ, Suttles J, Ahle JC, Simandle S, Fagoaga OR, Nehlsen-Cannarella SL (1995). Immune function in marathon runners versus sedentary controls. Med Sci Sports Exerc 27: 986 -92.

Ostojic SM, Ahmetovic Z (2009). Indicators of iron status in elite soccer players during the sports season. Int J Lab Hematol 31: 447-52.

Pedersen BK (1991). Influence of physical activity on the cellular immune system: mechanisms of action. Int J Sports Med 12(suppl): 23-9.

Pyne DB, Baker MS, Fricker PA, McDonald WA, Telford RD, Weidemann MJ (1995) Effects of an intensive 12-wk training program by elite swimmers on neutrophil oxidative activity. Med Sci Sports Exerc, 27(4):536-542

Rietjens GJ, Kuipers H, Adam JJ, Saris WH, Van Breda E, Van Hamont D, Keizer HA :(2005). Physiological, biochemical and psychological markers of strenuous training-induced fatigue. Int J Sports Med 26: 16-26.

Rietjens GJ, Kuipers H, Hartgens F, Keizer HA (2002). Red blood cell profile of elite Olympic distance triathletes. A three-year follow-up. Int J Sports Med 23: 391-6.

Rodrigues dos Santos JA, Candeias J, Magalhães MC (2006). Immunological and antropometric alterations induced by a kayaking ultramarathon. A case study. Revista Portuguesa de Ciências do Desporto 6: 143-53.

Santhiago V, Silva AS, Papoti M, Gobatto CA (2009). Responses of hematological parameters and aerobic performance of elite men and women swimmers during a 14-week training program. J Strength Cond Res 23: 1097-10

Sawka MN, Convertino VA, Eichner RE, Schnieder SM, Young AJ (2000). Blood volume: importance and adaptations to exercise training, environmental stresses, and trauma/sickness. Med Sci, Sports Exerc 32: 332-48.

Schumacher YO, Sahm D, Baumstark MW, Pottgiesser T (2010). Reticulocytes in athletes: Longitudinal aspects and the influence of long- and short-term exercise. Drug Test Anal 2: 469 -74.

Shaskey DJ, Green GA (2000). Sports haematology. Sports Med 29: 27-38.

Shimuzu K, Suzuki N, Imai T, Aizawa K, Nanba H, Hanaoka Y, Kuno S, Mesaki N, Kono I, Akama T (2011) Monocyte and T-cell responses to exercise training in elderly subjects. J Strength Cond Res, 25(9):2565-2572

Syu GD, Chen HI, Jen CJ (2012) Differential effects of acute and chronic exercise on human neutrophil functions. Med Sci Sports Exerc, 44(6):1021-1027

Smith JA, Martin DT, Telford RD, Ballas SK :(1999). Greater erythrocyte deformability in world-class endurance athletes. Am J Physiol 276 (Heart Circ Physiol 45) 2188-93.

Starkie RL, Rolland J, Angus DJ, Anderson MJ, Febbraio MA (2001). Circulating monocytes are not the source of elevations in plasma IL-6 and TNF-alpha levels after prolonged running. Am J Physiol Cell Physiol 280: 769 -74.

Telford RD, Sly GJ, Hahn AG, Cunningham RB, Bryant C, Smith JA (2003). Footstrike is the major cause of hemolysis during running. J Appl Physiol 94: 38-42.

Vergès S, Devouassoux G, Flore P, Rossini E, Fior-Gozlan M, Levy P, Wuyam B (2005). Bronchial hyperresponsiveness, airway inflammation, and airflow limitation in endurance athletes. Chest 127: 1935-41.

Wahl P, Zinner C, Achtzehn S, Bloch W, Mester J (2010). Effect of high- and low-intensity exercise and metabolic acidosis on levels of GH, IGF-1, IGFBP-3 and cortisol. Growth Horm IGF Res 20: 380-5.

Watson RR, Moriguchi S, Jackson JC, Turner L, Wilmore J, Freund BJ (1988). Modification of cellular immune functions in humans by endurance exercise training during beta-adrenergic blockade with atenolol or propranolol. Med Sci Sports Exerc 18: 95-100.

Wu HJ, Chen KT, Shee BW, Chang HC, Huang YJ, Yang RS (2004). Effects of 24 h ultra-marathon on biochemical and haematological parameters. World J Gastroenterol 10: 2711 -4.




Cómo citar

Rodrigues dos Santos, J. A. (2019). Increasing running volume elicits hematological changes in trained endurance runners: a case study (El aumento del volumen de entrenamiento de carrera induce alteraciones hematológicas en corredores entrenados: un estudio de caso). Retos, 35, 117–120.



Artículos de carácter científico: trabajos de investigaciones básicas y/o aplicadas