Associação entre treinamento físico e açaí na homeostase do cálcio e inflamação no coração de ratos submetidos a dieta hiperlipídica

Victor  Neiva Lavorato; Denise  Coutinho de Miranda; Luciano Bernardes Leite; Marcelo  Eustáquio Silva; Antônio  José Natali

doi:10.47197/retos.v55.103103

Autores/as

Victor Neiva Lavorato Departamento de Educación Física, Centro Universitario Gobernador Ozanam Coelho (UNIFAGOC), Ubá, Minas Gerais, Brasil. https://orcid.org/0000-0001-9914-4722
Denise Coutinho de Miranda Departamento de Educación Física, Centro Universitario Gobernador Ozanam Coelho (UNIFAGOC), Ubá, Minas Gerais, Brasil. https://orcid.org/0000-0001-8714-6309
Luciano Bernardes Leite Departamento de Educación Física, Laboratorio de Biología del Ejercicio Universidad Federal de Viçosa, Viçosa, Brasil
Marcelo Eustáquio Silva Escuela de Nutrición, Universidad Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brasil
Antônio José Natali Departamento de Educación Física, Laboratorio de Biología del Ejercicio Universidad Federal de Viçosa, Viçosa, Brasil https://orcid.org/0000-0002-4927-4024

DOI:

https://doi.org/10.47197/retos.v55.103103

Palabras clave:

Entrenamiento de ejercicio; açaí; Contractilidad; Inflamación; Corazón.

Resumen

El objetivo del presente estudio fue investigar los efectos de la asociación de tratamientos con entrenamiento con ejercicios aeróbicos (AET) y suplementación con açai sobre la homeostasis del calcio (Ca²⁺) y los marcadores inflamatorios del corazón de ratas sometidas a una dieta rica en grasas. Se utilizaron ratas Fisher, divididas en cinco grupos experimentales: Control (C), Dieta Alta en Grasas (H); Dieta alta en grasas + Açai (HA); Dieta alta en grasas + AET (HT); Dieta alta en grasas + Açai + AET (HAT). Los grupos alimentados con una dieta rica en grasas recibieron 21,8% de manteca de cerdo y 1% de colesterol. Los grupos suplementados con açai liofilizado recibieron un 1% de açai en la dieta. Los animales de los grupos entrenados fueron sometidos a un programa de carrera progresiva en cinta rodante durante 8 semanas. Al final, se diseccionaron los corazones y se separaron los ventrículos izquierdos para el análisis de PCR en tiempo real. El grupo HT tuvo una mayor expresión genética del receptor de rianodina tipo 2 (RyR2) en comparación con el grupo H. En cuanto al intercambiador de sodio/calcio (NCX), el grupo H tuvo una menor expresión genética en comparación con los otros grupos. La interleucina 10 (IL-10) fue alta en el grupo HT en comparación con el grupo H. Los tratamientos cambiaron los parámetros de la homeostasis del Ca²⁺ y la inflamación, pero la asociación de intervenciones no proporcionó efectos adicionales.

Palabras clave: Entrenamiento de ejercicio; açai; Contractilidad; Inflamación; Corazón.

Abstract. The aim of the present study was to investigate the effects of the association of treatments with aerobic exercise training (AET) and açai supplementation on calcium homeostasis (Ca²⁺) and inflammatory markers of the heart of rats submitted to a high-fat diet. Fisher rats were used, divided into five experimental groups: Control (C), High-fat Diet (H); High-fat Diet + Açai (HA); High-fat Diet + AET (HT); High-fat Diet + Açai + AET (HAT). The groups fed a high-fat diet received 21.8% lard and 1% cholesterol. The groups supplemented with lyophilized açai received 1% of açai in the diet. The animals in the trained groups were submitted to a progressive treadmill running program for 8 weeks. At the end, the hearts were dissected, and the left ventricles separated for real-time PCR analysis. The HT group had higher gene expression of ryanodine receptor type 2 (RyR2) compared to group H. Regarding the sodium/calcium exchanger (NCX), group H had lower gene expression compared to the other groups. Interleukin 10 (IL-10) was high in group HT when compared to group H. The treatments changed parameters of Ca²⁺ homeostasis and inflammation, but the association of interventions did not provide additional effects.

Keywords: Exercise training; Açai; Contractility; Inflammation; Heart.

Citas

Benatti, F. B., & Pedersen, B. K. (2015). Exercise as an anti-inflammatory therapy for rheumatic diseases—myokine regulation. Nat Rev Rheumatol, 11(2), 86-97.

Bonomo, L. F., Silva, D. N., Boasquivis, P. F., Paiva, F. A., Guerra, J. F. d. C., Martins, T. A. F., ... Oliveira, R. P. (2014). Açai (Euterpe oleracea Mart.) modulates oxidative stress resistance in Caenorhabditis elegans by direct and indirect mechanisms. PloS one, 9(3), e89933.

Browning, J. D., & Horton, J. D. (2004). Molecular mediators of hepatic steatosis and liver injury. The Journal of clinical investigation, 114(2), 147–152.

Burke, L. M., Ross, M. L., Garvican-Lewis, L. A., Welvaert, M., Heikura, I. A., Forbes, S. G., Mirtschin, J. G., … Hawley, J. A. (2017). Low carbohydrate, high fat diet impairs exercise economy and negates the performance benefit from intensified training in elite race walkers. The Journal of physiology, 595(9), 2785–2807.

Carneiro-Júnior, M. A., Quintão-Júnior, J. F., Drummond, L. R., Lavorato, V. N., Drummond, F. R, da Cunha, D. N., Amadeu, M. A., ... Natali, A. J. (2013). The benefits of endurance training in cardiomyocyte function in hypertensive rats are reversed within four weeks of detraining. J Mol Cell Cardiol, 57, 119-28.

Chen, X., Li, H., Wang, K., Liang, X., Wang, W., Hu, X., Huang, Z., & Wang, Y. (2019). Aerobic Exercise Ameliorates Myocardial Inflammation, Fibrosis and Apoptosis in High-Fat-Diet Rats by Inhibiting P2X7 Purinergic Receptors. Frontiers in physiology, 10, 1286.

Drummond, F. R., Soares, L. L., Leal, T. F., Leite, L. B., Rezende, L. M. T., Fidelis, M. R., Lavorato, V. N., … Natali, A. J. (2023). Effects of voluntary running on the skeletal muscle of rats with pulmonary artery hypertension. Front. Physiol. 14, 1-7.

Gaillard, D., Passilly-Degrace, P., & Besnard, P. (2008). Molecular mechanisms of fat preference and overeating. Annals of the New York Academy of Sciences, 1141, 163–175.

Kemi, O. J., Ceci, M., Condorelli, G., Smith, G. L., & Wisloff, U. (2008). Myocardial sarcoplasmic reticulum Ca2+ ATPase function is increased by aerobic interval training. European journal of cardiovascular prevention and rehabilitation: official journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology, 15(2), 145–148.

Kemi, O. J., Ellingsen, O., Smith, G. L., & Wisloff, U. (2008). Exercise-induced changes in calcium handling in left ventricular cardiomyocytes. Frontiers in bioscience: a journal and virtual library, 13, 356–368.

Kesherwani, V., Chavali, V., Hackfort, B. T., Tyagi, S. C., & Mishra, P. K. (2015). Exercise ameliorates high fat diet induced cardiac dysfunction by increasing interleukin 10. Frontiers in physiology, 6, 124.

Lavorato, V. N., Del Carlo, R. J., da Cunha, D. N., Okano, B. S., Belfort, F. G., de Freitas, J. S., da Mota, G. de F., ... Natali, A. J. (2016). Mesenchymal stem cell therapy associated with endurance exercise training: Effects on the structural and functional remodeling of infarcted rat hearts. J Mol Cell Cardiol, 90, 111-119.

Lavorato, V. N., Miranda, D. C., Isoldi, M. C., Drummond, F. R., Soares, L. L., Reis, E. C. C., Pelúzio, M. D. C. G., ... Natali, A. J. (2021). Effects of aerobic exercise training and açai supplementation on cardiac structure and function in rats submitted to a high-fat diet. Food research international (Ottawa, Ont.), 141, 110168.

Lima-Leopoldo, A. P., Leopoldo, A. S., da Silva, D. C., do Nascimento, A. F., de Campos, D. H., Luvizotto, R. A., de Deus, A. F., Freire, P. P., Medeiros, A., Okoshi, K., & Cicogna, A. C. (2014). Long-term obesity promotes alterations in diastolic function induced by reduction of phospholamban phosphorylation at serine-16 without affecting calcium handling. Journal of applied physiology, 117(6), 669–678.

Meléndez, G. C., McLarty, J. L., Levick, S. P., Du, Y., Janicki, J. S., & Brower, G. L. (2010). Interleukin 6 mediates myocardial fibrosis, concentric hypertrophy, and diastolic dysfunction in rats. Hypertension, 56(2), 225–231.

Natali, A. J., Turner, D. L., Harrison, S. M., & White, E. (2001). Regional effects of voluntary exercise on cell size and contraction-frequency responses in rat cardiac myocytes. The Journal of experimental biology, 204(Pt 6), 1191–1199.

Paulino, E. C., Ferreira, J. C., Bechara, L. R., Tsutsui, J. M., Mathias, W., Jr, Lima, F. B., Casarini, D. E., Cicogna, A. C., Brum, P. C., & Negrão, C. E. (2010). Exercise training and caloric restriction prevent reduction in cardiac Ca2+-handling protein profile in obese rats. Hypertension, 56(4), 629–635.

Sánchez, G., Araneda, F., Peña, J. P., Finkelstein, J. P., Riquelme, J. A., Montecinos, L., Barrientos, G., ... Donoso, P. (2018). High-Fat-Diet-Induced Obesity Produces Spontaneous Ventricular Arrhythmias and Increases the Activity of Ryanodine Receptors in Mice. International journal of molecular sciences, 19(2), 533.

Pereira, R. R., de Abreu, I. C., Guerra, J. F., Lage, N. N., Lopes, J. M., Silva, M., de Lima, W. G., Silva, M. E., & Pedrosa, M. L. (2016). Açai (Euterpe oleracea Mart.) Upregulates Paraoxonase 1 Gene Expression and Activity with Concomitant Reduction of Hepatic Steatosis in High-Fat Diet-Fed Rats. Oxidative medicine and cellular longevity, 2016, 8379105.

Reeves, P. G., Nielsen, F. H., & Fahey, G. C., Jr (1993). AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. The Journal of nutrition, 123(11), 1939–1951.

Schauss, A. G., Wu, X., Prior, R. L., Ou, B., Huang, D., Owens, J., Agarwal, A., … Shanbrom, E. (2006). Antioxidant capacity and other bioactivities of the freeze-dried Amazonian palm berry, Euterpe oleracea mart. (acai). Journal of agricultural and food chemistry, 54(22), 8604–8610.

Silveira, A. C., Fernandes, T., Soci, Ú. P. R., Gomes, J. L. P., Barretti, D. L., Mota, G. G. F., Negrão, C. E., & Oliveira, E. M. (2017). Exercise Training Restores Cardiac MicroRNA-1 and MicroRNA-29c to Nonpathological Levels in Obese Rats. Oxidative medicine and cellular longevity, 2017, 1549014.

VanWagner, L. B., Wilcox, J. E., Colangelo, L. A., Lloyd-Jones, D. M., Carr, J. J., Lima, J. A., Lewis, C. E., Rinella, M. E., & Shah, S. J. (2015). Association of nonalcoholic fatty liver disease with subclinical myocardial remodeling and dysfunction: A population-based study. Hepatology, 62(3), 773–783.

Wang, Z., Li, L., Zhao, H., Peng, S., & Zuo, Z. (2015). Chronic high fat diet induces cardiac hypertrophy and fibrosis in mice. Metabolism: clinical and experimental, 64(8), 917–925.

Zapata-Sudo, G., da Silva, J. S., Pereira, S. L., Souza, P. J., de Moura, R. S., & Sudo, R. T. (2014). Oral treatment with Euterpe oleracea Mart. (açaí) extract improves cardiac dysfunction and exercise intolerance in rats subjected to myocardial infarction. BMC complementary and alternative medicine, 14, 227.

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