Evaluation of Mutagenic Activity in Drinking Water through Ames Test

Abstract

Background: The sources of potencial mutagens in our environment are many, but the most important of these is water for public consumption. This is a result of the chlorinating process which is the main reason for the appearance of these mutagens. With this in mind, the aim of our study was to check a possible mutagenic activity, using the Ames test, in organic concentrates taken from water for public consumption in Madrid. Methods: Severa1 bacteria1 strains were used, namely Salmonella histidine dependent TA1535, TA1538, TA98 and TA100, taken originally from Salmonella typhimurium LT2. Each test was performed twice, with or without the introduction of the mammalian-microsome activation (S9 mix), as per the indications in Ames. The plate incorporation assay was used to test the mutagenicity. Al1 samples of the water in question were processed and treated so as to create concentrates of organic chlorinated compounds. Results: The highest levels of mutagenicity appeared in the TA1535 strain and in the tests where the microsome fraction was not used (IM=1,94). Conclusions: With regard to mutagenic evaluation in organic concentrates taken from water for public consumption, no positive activity was found in any of the tester strains.